Standard Swedish standard · SS-EN ISO 20418-1:2018

Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 1: Peptide detection using LC-ESI-MS with protein reduction (ISO 20418-1:2018)

Status: Valid

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Standard Swedish standard · SS-EN ISO 20418-1:2018

Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 1: Peptide detection using LC-ESI-MS with protein reduction (ISO 20418-1:2018)
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Scope
ISO 20418-1:2018 specifies a qualitative and quantitative testing method to determine the content of wool, cashmere, yak fibres and their blends in textiles by microscope preliminary screening, protein extraction, enzymatic digestion and specific peptides detection using a liquid chromatography-mass spectrometer equipped with electrospray ionization source (LCI-ESI-MC).
This method can be applied to relevant textile products at each process stage (i.e. from raw material to garment) with a homogeneous distribution of the components. It can be applied to different types of textile materials (e.g. staples, tops, yarns and fabrics) that contain wool, cashmere or yak fibres and their blends. The method is based on a preliminary identification of all fibres in the blend on the basis of their morphology, by light microscopy. The proteins are then extracted by a thiourea/urea/dithiothreitol (DTT) solution. An enzymatic digestion by trypsin of the protein extracted from the fibres is carried out. Analysis of the specific markers is performed by LC-MS and the percent composition is calculated.
This method is applicable to samples containing other kinds of fibres than wool, cashmere and yak, by combining its results with the results obtained using the ISO 1833 series and/or the ISO 17751 series.
ISO 20418-1:2018 does not apply if fibres of the same animal species are present (e.g. blends of cashmere and mohair); in this case, the quantitative analysis can be performed using microscopic analysis (e.g. ISO 17751 series).

Subjects

General (59.060.01)


Buy this standard

Standard Swedish standard · SS-EN ISO 20418-1:2018

Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 1: Peptide detection using LC-ESI-MS with protein reduction (ISO 20418-1:2018)
Subscribe on standards - Read more Dölj
Price: 875 SEK
standard ikon pdf

PDF

Price: 875 SEK
standard ikon

Paper

Price: 1 400 SEK
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Product information

Language: English

Written by: Svenska institutet för standarder

International title:

Article no: STD-80004216

Edition: 1

Approved: 5/22/2018

No of pages: 24